Ert2 Gene / Induction Of Cre Mediated Recombination From The Cre Ert2 Transgene 29 Download Scientific Diagram

Up to 10 cash back Mice harboring Cre ERT2 mTmG gene were left untreated or treated with tamoxifen or vehicle at 8 1 weeks of age. The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor.

Strategy To Knock In Creert2 Into The Mouse Nex1 Gene A 1 Download Scientific Diagram

Cre-ERT2 production was detected in tissues containing melanocytes.

Ert2 gene. Thus administration of estrogen receptor modulators enables temporal or inducible control of Cre activity and gene deletion. Which does not bind its natural ligand 17β-estradiol at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen OHT or tamoxifen and with lesser sensitivity ICI 182780. Which does not bind its natural ligand 17β-estradiol at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen OHT and with lesser sensitivity ICI 182780.

CreERT2 mouse models are particularly useful in vascular biology where constitutive gene deletion often causes lethality early in development. After tamoxifen induction at various times during embryogenesis and adulthood in a Cre-responsive reporter mouse strain genetic recombination was detected in the melanoblasts and melanocytes of the skin. In response to the often-asked question what.

This construct permits tamoxifen-dependent Cre activity. T Mice harboring Ece1 floxflox allele were intercrossed with Cre ERT2 Ece1 Δ mice to generate the experimental animals. Gene deletion of Mkk4 and Mkk7 in inducible double KO adult mice Actin-Cre ERT2 Cre Mkk4 floxflox Mkk7f loxflox.

Cre-ERT2 contains Cre fused to the ligand-binding domain of a mutated estrogen receptor which recognizes tamoxifen or its derivative 4-hydroxytamoxifen4-OHT. The ERT2 sequence is an estrogen receptor targeting motif whose nuclear translocation and activity are controlled by estrogen receptor modulators. Cre ERT2 is a recombinant fusion protein of the Cre DNA recombinase and a mutant ligand-binding domain of the human estrogen receptor.

This protein is found in a cell structure called the Golgi apparatus which modifies newly produced enzymes and other proteins. This plasmid is available through Addgene. The administration of tamoxifen in these 2-month-old mice induced the gene deletion Actin-Cre ERT2 Cre Mkk4 Mkk7 genotype which was verified by PCR Western blot and immunohistochemistry techniques.

ERT2 - ERT2 protein - Arabidopsis thaliana Mouse-ear cress - ERT2 gene protein UniProtKB - O49747 O49747_ARATH. The transgene integrated into chromosome 2 causing a 5 bp deletion in Ndor1 NADPH dependent diflavin oxidoreductase 1. Here we detail the most cited pan-endothelial inducible Cre mouse alleles with an emphasis on recombination specificity timing and recombination efficiency.

B A schematic showing the generation of inducible Ece1 KO mice. TSC1 deletion was detected in the lungs of tamoxifen treated SPC-Cre-ER T2TSC1 fxfx mice. Detailed Description These transgenic mice express a Cre-ERT2 fusion gene under the control of the human ubiquitin C UBC promoter.

A B Representative agarose gel electr ophoresis of PCR for. Kryn Stankunass lab contains the insert ERT2-CRE-ERT2 and is published in PLoS One. The organspecific and temporal control of gene activationinactivation is a key issue in the understanding of protein function during normal and pathological development and during oncogenesis.

These are also summarized in the Table. The endogenous locus was modified by insertion into the first intron of a construct containing the rabbit beta-globin 3 splice site followed by the creERT2 fusion gene which encodes Cre recombinase joined to a mutant hormone-binding domain of the human estrogen receptor that is activated by 4-hydroxytamoxifen 4-OHT but not by the natural ER ligand 17-beta-estradiol. To achieve this goal Actin-Cre ERT2 Cre Mkk4 floxflox Mkk7 floxflox mice were created.

Plasmid pME-ERT2-Cre-ERT2 from Dr. Please note that Addgenes sequencing results identified a K183E mutation in the Cre protein sequence. We generated transgenic mice bearing a tamoxifendependent Cre recombinase TyrCreERT2 gene expressed under the control of a 61 kb murine tyrosinase promoter in order to.

Therefore this SPC-Cre-ER T2 mouse model may be a valuable tool to investigate functions of genes in lung development physiology and disease. Mice hemizygous for this Cre-ERT2 transgene are viable and fertile. The EXT2 gene provides instructions for producing a protein called exostosin-2.

This method allows one to control gene activity in space and time in almost any tissue of the mouse thus opening new avenues for studying gene function and for establishing sophisticated animal models of human diseases. The Cre-ERT2 fusion protein Cre-ERT2 consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor. The Crelox site-specific recombination system has emerged as an important tool for the generation of conditional somatic mouse mutants.

Similarly to the endogenous estrogen receptor Cre ERT2. Other lines allow for lineage tracing of Wnt responding cells using the CreERT2 gene inserted into Wnt target genes.

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